发明名称 |
SEQUENCE-SPECIFIC ENGINEERED RIBONUCLEASE H AND THE METHOD FOR DETERMINING THE SEQUENCE PREFERENCE OF DNA-RNA HYBRID BINDING PROTEINS |
摘要 |
The subject of the invention is the ribonuclease which cleaves RNA strand in DNA-RNA hybrids, wherein ribonuclease comprises fusion protein comprising catalytic domain of RNase HI (RNase HI) or derivative thereof with a zinc finger DNA-RNA hybrid binding domain, and wherein the zinc finger binding domain has the ability to bind to specific sequences in the DNA-RNA hybrid. The invention also relates to new methods for determination of the sequence preference of DNA-RNA hybrid binding protein(s) or its domain(s) and allows determining the sequence recognized by sequence specific binding protein in the DNA-RNA hybrid. |
申请公布号 |
US2014094385(A1) |
申请公布日期 |
2014.04.03 |
申请号 |
US201314095351 |
申请日期 |
2013.12.03 |
申请人 |
MIEDZYNARODOWY INSTYUT BIOLOGII MOLEKULARNEJ I KOMORKOWEJ |
发明人 |
BUJNICKI JANUSZ MAREK;SULEJ AGATA AGNIESZKA;SKOWRONEK KRZYSZTOF JERZY;NOWOTNY MARCIN |
分类号 |
C12N9/22;C07K14/435 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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