METHODS FOR IDENTIFYING INHIBITORS OF ABETA42 OLIGOMERS

摘要

The invention herein is directed to immunoassays for the detection of &Agr;&bgr;42 oligomers. The inventive assays are based on the observations herein that the presence of &Agr;&bgr;42 oligomers in a preparation is directly related to a decrease in a C-terminal (CT) immunosignal and a correlated increase in an N-terminal (NT) immunosignal, relative to the immunosignal generated in the absence of &Agr;&bgr;42 oligomers, in an &Agr;&bgr;42 CT and NT ELISA assay and an &Agr;&bgr;42 CT AlphaLISA assay. The invention herein involves the use of these assays alone or in combination to screen for inhibitors of &Agr;&bgr;42 oligomerization.