MICROFLUIDIC CHIP FOR MULTI-ANALYTE DETECTION

摘要

A microfluidic chip and compatible biosensor are provided to detect and/or quantify an analyte in a sample fluid, and preferably to simultaneously quantify multiple analyte(s) in a sample fluid volume. A fluid volume containing microbeads is flowed through an elongate planar sample chamber of the microfluidic chip. Microbead traps or barriers in the sample chamber retain the microbeads. The sample fluid volume is flowed through the sample chamber. A conjugate specific to the analyte is and labeled with a fluorophore is introduced into the sample chamber. In the biosensor, an excitation wavelength is directed parallel to the plane of the sample chamber. Any fluorescent emissions from the sample chamber are detected in a direction substantially orthogonal to the plane of the sample chamber by a photodetector, and correlated to an amount or concentration of analyte in the sample fluid volume.