发明名称 |
TWO-PRIMER PCR FOR MICRORNA MULTIPLEX ASSAY |
摘要 |
The present invention provides method for amplifying a specific RNA molecule in a sample, the method comprising: (a) adding a poly(ribonucleotide) sequence to RNA molecules in the sample; (b) reverse transcribing the poly-adenylated RNA molecules using a reverse primer comprising a sequence that anneals to said poly(ribonucleotide) sequence; and (c) amplifying and detecting the cDNA molecule(s) using the same reverse primer and using a forward primer specific for the RNA molecule to be detected; wherein at least one of the forward and reverse primers comprises a hairpin primer. The invention also provides kits useful for practicing this method. |
申请公布号 |
WO2014052487(A1) |
申请公布日期 |
2014.04.03 |
申请号 |
WO2013US61743 |
申请日期 |
2013.09.25 |
申请人 |
CEPHEID |
发明人 |
HIGUCHI, RUSSELL;LAI, EDWI,N WEI-LUNG;LOKHOV, SERGEY |
分类号 |
C12Q1/68;C12N15/11 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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