PRODUCTION METHOD OF THREE-DIMENSIONAL CELL CULTURE CONSTRUCT

摘要

PROBLEM TO BE SOLVED: To provide a new production method of a three-dimensional cell culture construct.SOLUTION: A production method of a three-dimensional cell culture construct in which at least two cell layers are laminated comprises: seeding a coated cell in which the surface is covered with a coating containing an extracellular matrix component; cultivating the seeded coated cell in a medium; and continuously using at least part of the medium for 5 or more days. Further, the production method of a three-dimensional cell culture construct in which at least two cell layers are laminated comprises: seeding a coated cell in which the surface is covered with a coating containing an extracellular matrix component; and cultivating the seeded coated cell in a medium, in which the coated cell is cultivated in 70 μL or more of the medium per 1×10cells.