PROKARYOTIC RNAi-LIKE SYSTEM AND METHODS OF USE

摘要

Provided herein are methods for inactivating a target polynucleotide. The methods use a psiRNA having a 5′region and a 3′region. The 5′region includes, but is not limited to, 5 to 10 nucleotides chosen from a repeat from a CRISPR locus immediately upstream of a spacer. The 3′region is substantially complementary to a portion of the target polynucleotide. The methods may be practiced in a prokaryotic microbe or in vitro. Also provided are polypeptides that have endonuclease activity in the presence of a psiRNA and a target polynucleotide, and methods for using the polypeptides.