发明名称 |
METHODS FOR DNA AND RNA EXTRACTION FROM FIXED PARAFFIN-EMBEDDED TISSUE SAMPLES |
摘要 |
<p>Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50C to about 100C; and recovering the nucleic acid from said lysis solution.</p> |
申请公布号 |
CA2886389(A1) |
申请公布日期 |
2014.04.03 |
申请号 |
CA20132886389 |
申请日期 |
2013.09.26 |
申请人 |
CEPHEID |
发明人 |
LAI, EDWIN WEI-LUNG;VANATTA, REUEL;HO, KENNETH E. |
分类号 |
C12N15/10;C12Q1/68;G01N1/28 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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