| 摘要 |
<p>The invention provides a method for expansion of limbal stem cells, comprising:(i) providing a limbal biopsy on a growth support;(ii) culturing for a first time period the limbal biopsy in the presence of epithelial culture medium supplemented with serum and one or more antibiotics; and(iii) substituting the epithelial culture medium of step ii) with epithelial culture medium which is supplemented with serum and which is free from antibiotics, and culturing for a second time period.</p> |
