METHOD FOR PREPARING VIRUS LIKE PARTICLE OF NODAVIRUS, YEAST STRAIN PRODUCING THE SAME AND VACCINE COMPOSITION COMPRISING THE SAME

摘要

The present invention relates to a method for producing virus-like particles of nodavirus, a yeast cell line expressing the same, and a vaccine composition containing the same. According to the method of the present invention, it is possible to mass produce high-quality virus-like particles of nodavirus by expressing a nodavirus capsid protein through a yeast expression system and purifying the same by chromatography. The virus-like particles of nodavirus prepared by the method of the present invention can be very useful as an antigen of a vaccine against nodavirus infection. In addition, the present invention provides a recombinant yeast strain expressing a nodavirus capsid protein, and a vaccine composition containing the recombinant yeast strain or a cultured product thereof.