| 摘要 |
Elastase is obtained from defatted pancreas by extraction with sodium acetate-acetic acid buffer at pH 4.8 to 5 followed by precipitation with (NH4)2SO4 at 48 to 55% saturation. The precipitate is resuspended in sodium acetate-acetic acid buffer and, after demineralization, e.g. by dialysis, it is fractionally precipitated from a water-miscible alcohol/water mixture. The active precipitate is recovered, cold-centrifuged, redissolved in sodium acetate-acetic acid buffer at pH 4.7 to 5 and the solution lyophilized. All operations are carried out at a temperature below 5 DEG C.ALSO:Elastase is obtained from defatted pancreas by extraction with sodium acetate-acetic acid buffer at pH 4.8 to 5 followed by precipitation with (NH4)2SO4 at 48 to 55% saturation. The precipitate is resuspended in sodium acetateacetic acid buffer and after demineralization, e.g. by dialysis, it is fractionally precipitated from a water-miscible alchol/water mixture. The active precipitate is recovered, cold-centrifuged, redissolved in sodium acetate-acetic acid buffer at pH 4.7 to 5 and the solution lyophilized. All operations are carried out at a temperature below 5 DEG C.
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