| 摘要 |
Plasminogen is prepared from animal blood plasma or serum by a process comprising the following steps in any suitable order: (i) addition of water, at a temperature between the freezing point of the mixture and 25 DEG C., so as to dilute the plasma or serum 1 to 4 times; (2) addition of sufficient organic solvent such that a final mixture of water, plasma or serum, and solvent will contain 2 to 10% by volume of the latter; (3) arranging that the pH of the final mixture will lie between 4 and 7; and finally separating the plasminogen-containing precipitate from the mixture. Organic solvents may be water-miscible, e.g. ethanol, methanol, dioxane or acetone, or only slightly miscible, e.g. ether. The plasma is prepared by citrating fresh blood, if desired adding an antibiotic such as penicillin or streptomycin, centrifuging off the corpuscles and then re-calcifying. This may be converted to serum by vigorously stirring for several hours and separating the precipitated fibrin. The plasminogen prepared may be used, after activation to plasmin by treatment with trypsin or urokinase, for clinical applications (see Divisions A5-A6).ALSO:Plasminogen is prepared from animal blood plasma or serum by a process comprising the following steps in any suitable order: 1) Addition of sufficient water, at a temperature between the freezing point of the mixture and 25 DEG C, to dilute the plasma or serum 1 to 4 times; 2) Addition of sufficient organic solvent to provide a final concentration thereof in the mixture of 2 to 10% by volume; 3) Arranging that the pH of the final mixture lies between 4 and 7; and finally separating the resulting plasminogen containing precipitate from the mixture. Organic solvents may be water-miscible, such as acetone, ethanol, methanol, or dioxane, or only slightly miscible, such as ether. Suitable animal blood is that from the pig or ox, which is freshly collected, citrated, centrifuged to remove corpuscles and re-calcified to provide plasma, or the plasma stirred vigorously to precipitate fibrin and provide serum. The plasminogen is converted to plasmin by treatment with urokinase or trypsin.
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