| 摘要 |
This invention provides a cell growth medium comprising (a) a human platelet lysate free of solid matter greater than 0.22μm in diameter; (b) a human fresh frozen plasma (FFP) filtrate free of solid matter greater than 0.22μm in diameter; (c) heparin; (d) L-glutamine; and (e) a serum-free, low glucose medium suitable for mammalian cell growth, wherein the cell growth medium permits the expansion of human CD34−stem cells and wherein the resulting expanded CD34−stem cells retain the ability to differentiate. This invention also provides related cell growth medium supplements, a sterile human platelet lysate and human fresh frozen plasma (FFP) filtrate, kits, CD34−stem cell-containing compositions, and related production and cell expansion methods. |
