| 摘要 |
2-Ketogluconic acid is prepared by the microbiological oxidation of glucose, whereby a nutrient medium having a pH of about 6.5 and containing 18-30% by weight glucose and an assimilable source of nitrogen is inoculated with a growing culture of a microorganism selected from the Pseudomonas genus and fermentation allowed to proceed whilst aerating the medium and adjusting the pH to 5-7 during the active phase growth of the microorganism by addition of ammonia into the nutrient medium. The ammonia may be added as the gas, liquid or in aqueous solution. Corn steep liquor is the preferred source of nitrogen. The 2-ketogluconic acid may be isolated as the ammonium salt and converted to the free acid, e.g. on a cation exchange resin column.
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