| 摘要 |
FIELD: veterinary.SUBSTANCE: method for detecting bird's dysbiosis is characterised by the fact that it provides for use of a detection device of an electronic nose type based on a block of 8 piezosensors with basis oscillation frequency of 10-15 MHz, the electrodes of which are modified with coatings sensitive to nitrogen-containing organic compounds, aliphatic acids, compound ethers, aromatic and aliphatic amines, amino acids, ammonium, sulphur-containing compounds, and water. At investigation of birds by means of the detection device of the electronic nose type for dysbiosis of their bowels, first, total content of gases-markers of the disease is determined; for that purpose, average sample of the bird's manure with the weight of 5.00 g is placed into a sterile glass vessel with a soft membrane, thermally regulated at the temperature of 25°C during 20 minutes, extracted with an individual syringe (5 cm) of an equilibrium gas phase and introduced to a detection cell; maximum signals of the block of piezosensors are recorded by means of the programme. After that, a compressor is switched on for 1-2 minutes to clean the detection cell and the piezosensors. Then, a provisional index of dysbiosis is calculated as a ratio of maximum signals of sensors with films of polyvinyl pyrrolidone (PVP) to polydiethyleneglycol succinate (PDEGS), which reflect the ratio of content of free water to amines in equilibrium gas phase above the sample if the dysbiosis index is less than 1.10±0.05; a conclusion is made on availability of dysbiosis state of birds; with that, maximum signals of other sensors in the block, which are related to content of other gases-markers such as compound ethers, aliphatic acids and amino acids, sulphur-containing compounds, shall be lower in equilibrium gas phase than signals of water-detecting sensors, with a polyvinyl pyrrolidone coating, and amines, with a polydiethyleneglycol succinate coating; with that, content of individual classes of compounds ("?") is calculated by a standardisation method.EFFECT: improving accuracy and reliability, diagnostics informativity as per a provisional dysbiosis index - the ratio of content of free water and amines; detection of different classes of organic substances and equilibrium gas phase above manure samples is simplified.3 tbl |
