| 摘要 |
The probability of extracting a region containing a large amount of fluorescent material is increased in accordance with the kind of fluorescent reagent etc. A fluorescence observation device includes an illuminating unit irradiating a subject with excitation light and illumination light; a fluorescence image capturing unit acquiring a fluorescence image by capturing fluorescence generated at the subject a return-light image capturing unit acquiring a return-light image by capturing return light returned from the subject; an extracting unit determining and extracting a region having a gray level exceeding a gray-level threshold from the fluorescence image as a region-of-interest; a display unit displaying the region-of-interest and the return-light image in association with each other; a true/false input unit prompting an observer to enter true or false as to the determination of the region-of-interest; and a threshold updating unit updating the gray-level threshold so as to reflect the input result entered. |
