METHOD FOR EXPRESSING, EXTRACTING AND REFINING SOLUBLE RECOMBINANT PROTEIN

摘要

The present invention relates to a method for expressing, extracting and refining recombinant human growth hormone (hGH). More particularly, the present invention relates to an intracellular expression method capable of minimizing the formation of insoluble inclusion bodies during mass production of protein in Escherichia coli systems. The present invention also relates to an extraction and refining method capable of maximizing solubility during extraction of a target protein, in particular human growth hormone, thus achieving biological activity and high yield ratio.