| 摘要 |
PROBLEM TO BE SOLVED: To enable detection of high sensitivity on a monomolecular level in a detection method for detecting the amount of a substance to be detected. SOLUTION: A sample S is supplied to a sensor part 14 containing a metal layer 12 and the sensor part 14 is irradiated with exciting light Lo to produce an intensified photoelectric field D on the sensor part 14 and a fluorescent label is excited to detect the amount of the substance A to be detected on the basis of the quantity of the light caused by this excitation. In this detection method, a fluorescent substance F obtained by including a plurality of fluorescent coloring matter molecules (f) and at least one fluorescence intensifying fine particle M by a light pervious material 16 for transmitting the fluorescence produced from a plurality of the fluorescent coloring matter molecules (f) is used as the fluorescent label. The fluorescence Lf produced from the fluorescent label is increased by the interaction or the like of the fluorescence intensifying fine particle M with the photoelectric field D, the metal layer 12 or the other fluorescence intensifying fine particle M provided in the vicinity. COPYRIGHT: (C)2010,JPO&INPIT |
