METHODS FOR PRODUCING RECOMBINANT FACTOR VIII CHAINS FROM NON-FILAMENTOUS FUNGI, THEIR FUNCTIONAL RECONSTITUTION AND APPLICATIONS THEREOF

摘要

The present disclosure relates specifically to a process of producing heavy chain peptide and/or light chain peptide of recombinant Factor VIII protein using Pichia pastoris expression system. The disclosure further relates to a process of producing a functional recombinant Factor VIII protein by reconstituting the Heavy chain and Light chain produced using said Pichia pastoris expression system. The said functional recombinant Factor VIII protein shows improved activity and therefore is used in the management of haemophilia.