| 摘要 |
FIELD: chemistry.SUBSTANCE: invention relates to biochemistry, particularly to an isolated version of a Bowman Birk protease inhibitor (BBPI), where said BBPI version has a frame containing amino acid sequences 1-40 and 50-66 of SEQ ID NO: 187 and cysteine residues in amino acid positions 41 and 49 of SEQ ID NO: 187, between which is included a binding peptide selected from a binding VEGF peptide, a binding FGF-5 peptide, a binding TGFb peptide and a binding TNF? peptide, where said BBPI version further includes a substituted amino acid in an amino acid position selected from positions equivalent to positions 1, 4, 5, 11, 13, 18, 25, 27, 29, 31, 38, 40, 50, 52, 55 and 65 of SEQ ID NO: 187, where said substituted amino acid in position 1 is A, in position 4 is V, in position 5 is selected from P and A, in position 11 is 11G, in position 13 is selected from 13Y, 13I, 13F, 13M, 13L, 13V, 13K and 13R, in position 18 is selected from 18I and 18V, in position 25 is selected from 25K, 25N, 25W, 25I, 25A and 25R, in position 27 is selected from 27H, 27K, 27V, 27A and 27Q, in position 29 is selected from 29R, 29K and 29P, in position 31 is selected from 31Q, 31H, 31E, 31A, 31R, 31W, 31K and 31T, in position 38 is selected from 38N, 38K and 38R, in position 40 is selected from 40H, 40K, 40Q, 40R and 40Y, in position 50 is selected from 50R, 50Q, 50K, 50T, 50V, 50M and 50S, in position 52 is selected from 52K, 52T, 52R, 52Q, 52L, 52H, 52A, 52M, 52S and 52E, in position 55 is 55M, in position 65 is selected from 65E and 65D.EFFECT: invention enables to effectively inhibit trypsin compared with a non-modified variant.6 cl, 18 dwg, 7 tbl, 18 ex |
