摘要 |
The present invention relates to a method for increasing the binding reversibility of a omega-conotoxin to a N-type calcium channel, which comprises preparing a omega-conotoxin having a Ile and/or Ala residue at a position of amino acid (11 and/or 12), respectively in the second loop between cysteine residues (2 and 3) of the omega-conotoxin represented by the formula I, such that the prepared omega-conotoxin has the increased binding reversibility to N-type calcium channel. In addition, the present invention relates to a novel omega-conotoxin and a pharmaceutical composition having plausible properties in view of blocking activity to and specificity to N-type calcium channel, and dramatically improved binding reversibility to N-type calcium channel. |