发明名称 |
Métodos para avaliar células malignas em uma epiderme in vitro, e para avaliar compostos de teste quanto à toxicidade humana. |
摘要 |
A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis. |
申请公布号 |
BR9912047(B1) |
申请公布日期 |
2014.03.18 |
申请号 |
BR19999912047 |
申请日期 |
1999.07.12 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
LYNN ALLEN-HOFFMANN;SANDY J. SCHLOSSER;MICHAEL A. PICKART |
分类号 |
G01N33/50;A61P35/00;A61P43/00;C12N1/00;C12N5/071;C12N5/10;C12N15/09;C12Q1/02;G01N33/15 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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