摘要 |
Methods for making expression constructs for enhancing gene expression in an organism are provided. The expression constructs comprise a constitutive promoter operably linked to a polynucleotide, which comprises a 5'-untranslated region (5'-UTR) and a translated region. The 5'-UTR comprises the first intron of a gene that is native to an organism and expressed constitutively. Methods of using the expression constructs to enhance the expression of a gene in an organism and compositions comprising the expression constructs are further provided. |