ENGINEERED YEAST FOR PRODUCTION OF RENEWABLE CHEMICALS

摘要

The present application relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial, renewable metabolites, e.g., isobutanol. In some embodiments, the recombinant yeast microorganisms may be engineered to comprise an isobutanol producing metabolic pathway, wherein said isobutanol producing metabolic pathway comprises at least one exogenous gene and/or at least one overexpressed endogenous gene encoding an enzyme that catalyzes a pathway step in the conversion of pyruvate to isobutanol. In certain exemplary embodiments, said recombinant yeast microorganisms may additionally or independently be engineered to alter the regulation, expression, and/or activity of a Mac1 pathway component.