发明名称 |
METHODS AND COMPOSITION FOR DETECTING INTESTINAL CELL-BARRIER DYSFUNCTION |
摘要 |
Methods for detecting intestinal cell barrier dysfunction in a patient are disclosed. In one method, patient intestinal epithelial cells (lECs), oropharyngeal epithelial cells (OECs) or buccal epithelial cells (BECs) are stained with detectable probes specific against caspase-1 and caspase-3&7, and the cells are viewed for the presence of elevated levels of caspase-1, as evidence by a significantly higher ratio of caspase-1 marker to caspase-3&7, as an indicator of cell barrier dysfunction. In a second method, in situ images of a patient's lEC's, OECs or BECs are obtained by probe-based confocal laser endomicroscopy (pCLE), and images are analyzed for density of cell gaps. Also disclosed is a probe composition for use in detecting intestinal cell barrier dysfunction. |
申请公布号 |
WO2014039699(A1) |
申请公布日期 |
2014.03.13 |
申请号 |
WO2013US58296 |
申请日期 |
2013.09.05 |
申请人 |
ARCH BIOPARTNERS INC.;IRVIN, RANDALL, THOMAS;LIU, JULIA;DAVIS, ELISABETH, MELIKA |
发明人 |
IRVIN, RANDALL, THOMAS;LIU, JULIA;DAVIS, ELISABETH, MELIKA |
分类号 |
C12Q1/37 |
主分类号 |
C12Q1/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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