摘要 |
PURPOSE: A redifferentiation method of Salix koreensis and a method for improving the same are provided to quickly proliferate selected male trees of Salix koreensis with excellent growth and to effectively supply Salix pseudo-lasiogyne and Salix caprea L. CONSTITUTION: A method for improving the redifferentiation ratio of Salix koreensis shoots comprises the steps of: culturing Salix koreensis by low light for two weeks; and culturing Salix koreensis by strong light for four weeks. The method comprises a pre-cultivation of the shoots in a medium containing 2.0 mg/L of 2ip ( (2-isopentenyl) adenine) and 0.1 mg/L of naphthalene acetic acid (NAA) for two weeks. The method further comprises the step of the shoots in a shoot-inducing medium containing 2.0mg/L of NAA-free 2ip. |