发明名称 |
Enzymatic substrate building blocks for multiple detection systems |
摘要 |
<p>An inventive substrate is provided which includes a substrate compound of formula A-B 1 -B 2 -B 3 -B 4 : wherein A is a sugar moiety; B 1 is a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; B 2 is a linker moiety with a free reactive amino group so as to be available for reaction with carboxylic acids or detectable tags; B 3 contains a permanently charged element such as a quaternary ammonium group so as to increase sensitivity for mass spectrometry analysis; and B 4 of various carbon length conferring specificity amongst individual substrates in detection methods. Also provided is a molecule of the formula B 1 -B 2 -B 3 -B 4 , with similar structural characteristics to an enzymatic product produced by the action of a target enzyme on an inventive substrate. Further provided are methods for using inventive substrates for detecting enzymatic activity.</p> |
申请公布号 |
EP2706121(A1) |
申请公布日期 |
2014.03.12 |
申请号 |
EP20130184562 |
申请日期 |
2009.03.13 |
申请人 |
PERKINELMER HEALTH SCIENCES, INC. |
发明人 |
CERDA, BLAS;BOBROW, MARK |
分类号 |
C12Q1/34;C07C215/66;C07C229/00 |
主分类号 |
C12Q1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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