发明名称 |
DIAGNOSTICS METHOD OF SENSITIVITY OF Mycobacterium tuberculosis STRAINS TO INJECTION ANTITUBERCULOUS PREPARATIONS OF RESERVE ROW (AMINOGLYCOSIDES AND CAPREOMYCIN) |
摘要 |
FIELD: biotechnologies.SUBSTANCE: invention presents a diagnostics method of sensitivity of M. tuberculosis (MBT) to injection antituberculous preparations of a reserve row. The method involves a DNA extraction stage, amplification of the investigated DNA sections by means of a polymerase chain reaction method and analysis of conformational polymorphism of single-chain fragments (SSCP). Gene section rrs is amplified in 50 mcl of a reaction mixture with addition of 5 mcl of the specimen. Promoter gene section eis is amplified in 30 mcl of the reaction mixture containing direct 5'CGGAGCCGTCGGGGTATGC and reverse 5'GCCGCGGCCAGTAGGAACA primers and 3 mcl of the specimen as per the amplification programme: 1-st stage - 95°- 4 min; 2-nd stage - 95° - 20 sec, 59° - 30 sec, 72° - 20 sec (30 cycles); 3-rd stage: 72° - 4 min; 10° - storage. Separation of amplification products in the ratio of 4 mcl of the specimen and 6 mcl of a denaturing dye is performed by electrophoresis in 8% polyacrylamide gel with 5% glycerin at voltage of 400 Volts during 5 hours at 8°C. Gel painting is performed by means of caustic silver.EFFECT: invention allows diagnosing sensitivity of M tuberculosis to injection antituberculous preparations of a reserve row with high accuracy.2 dwg |
申请公布号 |
RU2509158(C2) |
申请公布日期 |
2014.03.10 |
申请号 |
RU20120110534 |
申请日期 |
2012.03.21 |
申请人 |
GOSUDARSTVENNOE KAZENNOE UCHREZHDENIE ZDRAVOOKHRANENIJA MOSKOVSKIJ GORODSKOJ NAUCHNO-PRAKTICHESKIJ TSENTR BOR'BY S TUBERKULEZOM DEPARTAMENTA ZDRAVOOKHRANENIJA G. MOSKVY |
发明人 |
GIKALO MARINA BORISOVNA;NOSOVA ELENA JUR'EVNA;KRASNOVA MARIJA ALEKSANDROVNA;BUKATINA ANASTASIJA ALEKSANDROVNA;GALKINA KSENIJA JUR'EVNA;MOROZ ARKADIJ MAKSOVICH;LITVINOV VITALIJ IL'ICH |
分类号 |
C12Q1/68;C12R1/32 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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