MYCOBACTERIUM COMPRISING EXPRESSION VECTOR WITH TWO AUXOTROPHIC SELECTION MARKERS AND ITS USE AS VACCINE

摘要

The invention relates to polynucleotides and recombinant cell strains comprising said polynucleotides and the uses thereof for the delivery of the polypeptides encoded by said polynucleotides to a subject in need thereof. In particular, the invention refers to polynucleotides comprising a polypeptide of interest, auxotrophy-complementing genes and the use thereof in a mycobacterial double auxotrophic host cell to achieve the stable expression of the polypeptide of interest by using an antibiotic- free plasmid selection system.