DEVICES FOR AUTOMATED SAMPLE COLLECTION, QUANTIFICATION, AND DETECTION FOR INSECT BORNE BIO-AGENT SURVEILLANCE

摘要

Devices and methods for insect surveillance are disclosed. In particular, the invention relates to devices for collecting samples of salivary fluid from insects and multiplexed high-throughput methods of screening insect samples for specific insect species, genetically modified strains, and insect-borne pathogens. An attractant is used to induce insects to deposit salivary droplets in a 2-dimensional microfluidic array having media-filled pockets sized to collect saliva from a single insect. The array for sample collection can be integrated with a microfluidic device for high-throughput processing of insect samples. The microfluidic device can be designed to perform multiplexed PCR or immunoassays, for example, for insect genotyping or pathogen detection. Devices can be used for screening populations of insects for arthropod-borne diseases, studying genetically modified populations released into the wild, determining the presence and quantity of specific arthropod species and pathogens, and delivery of bioagents.