发明名称 |
A PHARMACEUTICAL COMPOSITION FOR STIMULATION OF ANGIOGENESIS |
摘要 |
?Provided is a pharmaceutical composition for growth induction in blood vessel tissue which contains purified plasmid DNA encoding a vascular endothelial growth factor (VEGF) and pharmaceutically acceptable excipients that include a cryoprotectant as a vehicle and/or a pH stabilizer for stabilizing the pH in the range of 7.0 to 9.0, in effective quantities. Also provided is a storage method of plasmid DNA which encodes a VEGF comprising mixing the purified plasmid DNA with a solution of at least one cryoprotectant having properties of a vehicle and/or a pH stabilizer in the pH range of 7.0-9Ø The solution is lyophilized and stored at +2 to +8ºC. Supercoiled DNA pCMV-VEGF165 may be used which is produced by culturing Esherichia coli strain TOP10/pCMV-VEGF165. The pharmaceutical composition is administered to a human in quantities sufficient to provide a necessary therapeutic effect. The provided pharmaceutical composition of plasmid DNA pCMV-VEGF165 does not change significantly properties of the active substance when stored for a long time at a temperature from +2 to +8ºC. |
申请公布号 |
CA2881799(A1) |
申请公布日期 |
2014.03.06 |
申请号 |
CA20132881799 |
申请日期 |
2013.08.02 |
申请人 |
"NEXTGEN" COMPANY LIMITED |
发明人 |
KISELEV, SERGEJ L'VOVICH;DEEV, ROMAN VADIMOVICH;VOROB'EV, IVAN IVANOVICH;ORLOVA, NADEZHDA ALEKSANDROVNA;ISAEV, ARTUR ALEKSANDROVICH |
分类号 |
C12N1/21;A61K35/74;A61K38/18;A61P9/00;C07K14/435;C07K14/475;C12N15/09;C12N15/10;C12N15/12;C12N15/63 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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