ISOLATION OF STROMAL VASCULAR FRACTION FROM ADIPOSE TISSUE OBTAINED USING HOMOGENIZATION WITH BEADS

摘要

Methods of treating using adipose tissue using homogenization with beads to dissociate the fat cells and blood vessels contained within adipose tissue and thereby obtain stromal vascular fractions for use in human subjects are provided. These methods preferably do not include the use of any exogenous dissociating enzymes such as collagenase and result in increased numbers of the cells which constitute the stromal vascular fractions (about 10- fold greater) than methods which use collagenase to isolate these cells. Additionally, the adipose tissue may be isolated obtained from nonliving sources and animals (e.g., postmortem).