POLYMERASE CHAIN REACTION

摘要

A polymerase chain reaction (PCR) device for a reverse transcriptase reaction (RT) and a convectively-driven polymerase chain reaction (cPCR) in the same device is provided. The PCR device comprises an upper temperature-controlling unit, a middle temperature-controlling unit and a lower temperature-controlling unit. The middle temperature-controlling unit is used for controlling a temperature of a reaction mixture contained in a reaction container to have a temperature for the reverse transcriptase reaction. The middle temperature-controlling unit is disposed between the upper temperature-controlling unit and the lower temperature-controlling unit. The upper temperature-controlling unit and the lower temperature-controlling unit are used for simultaneously controlling the reaction mixture contained in the reaction container to have a temperature gradient and a convection condition for the convectively-driven polymerase chain reaction.