LIPASE VARIANTS HAVING INCREASED ENZYME SPECIFICITY OR ENHANCED TRANS-SELECTIVITY, AND METHODS OF USE

摘要

Disclosed are modified lipase enzymes, as well as polynucleotides and recombinant expression vectors encoding the lipase variant polypeptides, as well as methods for producing such lipase variants in selected bacterial and fungal host cells. The invention further relates to lipase variants having a preference for medium- or long- chain fatty acid esters or carboxylic acid moieties with carbon-chain lengths greater than or equal to C6. The invention further relates to methods for selectively reducing, or eliminating, one or more trans-fatty acids from substrates, including liquid edible oils useful in food manufacture and frying systems.