发明名称 |
Cell-free protein synthesis method with the use of linear template DNA and cell extract therefor |
摘要 |
It is provided a simple and efficient process for producing a large amount of protein in a cell-free protein synthesis system, through a continuous synthesis reaction over a long time using a linear DNA as a template. The continuous synthesis reaction is carried out for at least one hour with a cell-free protein synthesis system comprising an extract of Escherichia coli cells cultured at any temperature between 18° C. and 36° C., and a linear template DNA encoding the protein. The continuous protein synthesis system is preferably a dialysis system or a continuous flow system. The cell-free protein synthesis system by dialysis system comprises an internal dialysate comprising the extract and the linear template DNA, an external dialysate comprising a substrate solution for protein synthesis, and a dialysis membrane through which the substrate can be transferred. |
申请公布号 |
US8664355(B2) |
申请公布日期 |
2014.03.04 |
申请号 |
US20070798877 |
申请日期 |
2007.05.17 |
申请人 |
MATSUDA NATSUKO;KIGAWA TAKANORI;YOKOYAMA SHIGEYUKI;RIKEN |
发明人 |
MATSUDA NATSUKO;KIGAWA TAKANORI;YOKOYAMA SHIGEYUKI |
分类号 |
A61K38/00;C07K5/00;C07K7/00;C12P19/34;C12Q1/68 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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