发明名称 |
Rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of fermenting to produce fumaric acid |
摘要 |
A rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of producing fumaric acid by fermentation. The strain is named as Rhizopus oryzae ME-F13, and deposited in China Center for Type Culture Collection with depository number CCTCC M 2010351. The strain is obtained by physically mutagenizing the original strain ME-F12 through ion injection, culturing the processed bacteria on the solid selective plate containing 2-D-deoxylucose (2-DG) and picking up 2-DG-resistant single colony. The strain is capable of simultaneously saccharifying starchy material and fermenting it to produce fumaric acid. With an improved enzymatic activity, the strain can be directly used to ferment raw starchy materials without needing pre-saccharifying. |
申请公布号 |
US8663972(B2) |
申请公布日期 |
2014.03.04 |
申请号 |
US201113992233 |
申请日期 |
2011.04.16 |
申请人 |
HUANG HE;LI SHUANG;XU QING;GAO ZHEN |
发明人 |
HUANG HE;LI SHUANG;XU QING;GAO ZHEN |
分类号 |
C12N1/14;C12N15/01;C12P7/46;C12P19/20 |
主分类号 |
C12N1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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