发明名称 |
Probes for specific analysis of nucleic acids |
摘要 |
The present invention provides a method for detecting or enriching for a target deoxyribonucleic acid (DNA) present in a nucleic acid sample, said method comprising: (a) fragmenting a nucleic acid sample to generate nucleic acid fragments including a target fragment containing said target DNA and non-specifically ligating an adaptor sequence to an end of said fragments; (b) rendering said fragments at least partially single-stranded; (c) contacting the at least partially single-stranded fragments of step (b) with oligonucleotides A and B of a single target-specific nucleic acid probe; (d) ligating oligonucleotide B of said probe to the part of the single-stranded portion of said target fragment which is hybridised to oligonucleotide A of said probe to produce a probe-target fragment hybrid; and (e) detecting or enriching for said probe-target fragment hybrid. |
申请公布号 |
US8664164(B2) |
申请公布日期 |
2014.03.04 |
申请号 |
US201013384990 |
申请日期 |
2010.07.23 |
申请人 |
ERICSSON OLOF;ISAKSSON MAGNUS;JOHANSSON HENRIK;LANDEGREN ULF;AGILENT TECHNOLOGIES, INC. |
发明人 |
ERICSSON OLOF;ISAKSSON MAGNUS;JOHANSSON HENRIK;LANDEGREN ULF |
分类号 |
C12Q1/68;C12N15/09;C12Q1/44;C40B30/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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