Epsps mutants

摘要

Patent No. 597682 Disclosed is method for producing a non-transgenic, herbicide resistant or tolerant plant comprising: introducing into plant cells a recombinagenic oligonucleobase with a targeted mutation in the EPSPS gene to produce plant cells with a mutant EPSPS gene that expresses an EPSPS protein that is mutated at amino acid positions Thr179 and Pro183in an Arabidopsis EPSPS protein or at an analogous amino acid residue in an EPSPS homolog, wherein Thr179 is changed to Ile and Pro183 is changed to Thr or Ala; selecting a plant cell exhibiting improved tolerance to glyphosate as compared to a corresponding wild-type plant cell; and regenerating a non-transgenic herbicide resistant or tolerant plant having a mutated EPSPS gene from said selected plant cell.