发明名称 |
GENERATION METHOD OF ANTIGEN-SPECIFIC CYTOTOXIC CELLS WITH ACTIVITY AGAINST OVARIAN CARCINOMA CELLS |
摘要 |
FIELD: biotechnologies.SUBSTANCE: an in vitro generation method of antigen-specific cytotoxic cells with activity against ovarian carcinoma cells is proposed. Simultaneously, a non-adhesive fraction of mononuclear cells (MNC) and mature dendritic cells (DC) are cultivated in presence of recombinant human interleukine-12 and recombinant human interleukine-18. MNC are extracted from peripheral blood of patients having ovarian carcinoma. Mature DC are obtained from monocytes of adhesive MNC fraction after two-day cultivation, and first, activation by lysate of autologous ovarian carcinoma cells, and then maturation of DC loaded with lysate during one day in presence of recombinant human TMF-? (tumor necrosis factor).EFFECT: use of the invention provides reduction of a stage for obtaining mature DC, in vitro increases cytotoxicity of antigen-specific cytotoxic cells with antitumor activity and provides an immune response via T-helper to type 1 in respect to ovarian carcinoma, which can be used in ovarian carcinoma therapy.2 tbl |
申请公布号 |
RU2508298(C2) |
申请公布日期 |
2014.02.27 |
申请号 |
RU20120103822 |
申请日期 |
2012.02.03 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUT KLINICHESKOJ IMMUNOLOGII" SIBIRSKOGO OTDELENIJA ROSSIJSKOJ AKADEMII MEDITSINSKIKH NAUK (FGBU "NIIKI" SO RAMN) |
发明人 |
SENNIKOV SERGEJ VITAL'EVICH;OBLEUKHOVA IRINA ALEKSANDROVNA;KURILIN VASILIJ VASIL'EVICH;KRASIL'NIKOV SERGEJ EHDUARDOVICH;TARKHOV ALEKSANDR VALER'EVICH;GONCHAROV MAKSIM ALEKSANDROVICH |
分类号 |
C07K14/54;A61K39/00;A61P35/00;C12N5/0784 |
主分类号 |
C07K14/54 |
代理机构 |
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代理人 |
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