Provided is a method for producing ethanol with high efficiency through the ethanol fermentation from xylose using a saccharified biomass that contains various fermentation-inhibiting substances. A method for producing ethanol from a biomass according to the present invention comprises the steps of mixing xylose-utilizing yeast that has been so transformed as to over-express a gene for an acetic acid response transcription factor with a saccharified biomass and then culturing the yeast.
