| 摘要 |
<p>Microplates or microstrips having wells with extended length to receive immunosorbent elements protruding from a rod at the same modular distance of wells arranged in standard microstrips or microplates and methods of use are provided. A solid phase is constituted by the extended wells, each of which immobilize a different type of cell or molecule in the sample; the other solid phase is constituted by immunosorbent elements protruding from a rod, each of which has been previously coated with one of the same markers to be detected in the sample. Ligands for markers to be detected are added in a liquid phase to the wells; allowing the ligands competitively bind to the immunosorbent elements, inversely proportional to the quantity of the markers of each type of cell or molecule immobilized on the proper extended well. These ligands are simultaneously quantifiable by an immunoenzymatic assay using chromogenic substrate and a spectrophotometer.</p> |
