发明名称 |
Bisubstrate fluorescent probes for protein kinase CK2 |
摘要 |
<p>This invention relates to fluorescent probes for screening and characterization of compounds binding to protein kinase CK2, measurement of concentration of the catalytically active form of CK2 and imaging of CK2 activity in cells and tissues. The CK2-selective probe of the present invention interacts with binding sites of both substrates of the catalytic subunit of CK2 and therefore can be used for characterization of all inhibitors binding to the active site of CK2 in the binding/displacement assay. The high affinity of the probe affords the detection of the enzyme at low concentration and characterization of inhibitors in a wide affinity range.</p> |
申请公布号 |
EP2700946(A1) |
申请公布日期 |
2014.02.26 |
申请号 |
EP20120181818 |
申请日期 |
2012.08.24 |
申请人 |
UNIVERSITY OF TARTU |
发明人 |
URI, ASKO;VIHT, KAIDO;ENKVIST, ERKI |
分类号 |
G01N33/533;C07D235/04;C09B11/24;C09B23/08 |
主分类号 |
G01N33/533 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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