发明名称 |
Methods for detection of nucleotide modification |
摘要 |
This invention relates to the identification of modified cytosine residues, such as 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC) to be distinguished from cytosine (C) in a sample nucleotide sequence. Methods may comprise oxidising or reducing a first portion of polynucleotides which comprise the sample nucleotide sequence; treating the oxidised or reduced first portion and a second portion of polynucleotides with bisulfite; sequencing the polynucleotides in the first and second portions of the population following steps ii) and iii) to produce first and second nucleotide sequences, respectively and; identifying the residue in the first and second nucleotide sequences which corresponds to a cytosine residue in the sample nucleotide sequence. These methods may be useful, for example in the analysis of genomic DNA and/or of RNA. |
申请公布号 |
AU2012291873(A1) |
申请公布日期 |
2014.02.20 |
申请号 |
AU20120291873 |
申请日期 |
2012.07.27 |
申请人 |
CAMBRIDGE EPIGENETIX LIMITED |
发明人 |
BOOTH, MICHAEL JOHN;BALASUBRAMANIAN, SHANKAR |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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