发明名称 |
RECOMBINANT PLASMID DNA pCI-UB-POLYEPI CONTAINING EPITOPES OF TUMOUR-ASSOCIATED ANTIGENS FOR COLORECTAL CANCER AND METHOD OF ITS APPLICATION FOR STIMULATION OF SPECIFIC ANTITUMOUR IMMUNE RESPONSE AGAINST COLORECTAL CANCER CELLS |
摘要 |
FIELD: biotechnologies.SUBSTANCE: invention refers to creation of recombinant plasmids providing expression of poly-epitopic tumour-associated antigens in dendritic cells capable of stimulation of specific cytocidal cells, and it may be used in medicine. Recombinant plasmid DNA pCI-UB-POLYEPI contains 11 epitopes of tumour-associated antigens of colorectal cancer, its size is 6 355 n. p. and it expresses the following amino acid sequence: DYKDDDDK-LLGVGTFVV-ADRIW-GLKAGVIAV-AAYARY-VLAFGLLLA-ADRIW-YQLDPKFITSI-AAYARY-IMIGVLVGV-ADRIW-YLSGADLNL-AAYARY-CGIQNSVSA-AAYARY-LLLLTVLTV-ADRIW-QYIKANSKFIGlTEL-ANIY-SIINFEKL-ARY-SASFDGWATVSVIAL-ARY-SERVRTYWIIIELKHKARE-ARY-IQNDTGFYTLHVIKSDLVNEE. Mature dendritic cells obtained by adding to immature dendritic cells of pro-inflammatory TNF-? (tumour necrosis factor) cytokine are transfected by constructed plasmid DNA pCl-UB-POLYEPI thus activating them. Then activated dendritic cells are cultured together with peripheral mononuclear blood cells of people sick with colorectal cancer for generation of antigen-specific antitumour cytocidal cells.EFFECT: invention allows efficient generation of antigen-specific cytocidal cell with antitumour activity in vitro, required for immune response by the 1-st type T-helper to colorectal cancer antigens.2 cl, 1 dwg, 4 ex |
申请公布号 |
RU2507265(C2) |
申请公布日期 |
2014.02.20 |
申请号 |
RU20120119658 |
申请日期 |
2012.05.12 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUT KLINICHESKOJ IMMUNOLOGII" SIBIRSKOGO OTDELENIJA ROSSIJSKOJ AKADEMII MEDITSINSKIKH NAUK (FGBU "NIIKI" SO RAMN);FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "INSTITUT KHIMICHESKOJ BIOLOGII I FUNDAMENTAL'NOJ MEDITSINY" SIBIRSKOGO OTDELENIJA ROSSIJSKOJ AKADEMII NAUK (FGBU "IKHBFM" SO RAN) |
发明人 |
SENNIKOV SERGEJ VITAL'EVICH;FILIPPENKO MAKSIM LEONIDOVICH;KURILIN VASILIJ VASIL'EVICH;KULIKOVA EKATERINA VLADIMIROVNA;KHRAPOV EVGENIJ ALEKSANDROVICH;SHEVCHENKO JULIJA ALEKSANDROVNA;SHOROKHOV ROMAN VIKTOROVICH;JAKUSHENKO VLADIMIR KONSTANTINOVICH;SOKOLOV ANDREJ VIKTOROVICH |
分类号 |
C12N15/63;C12N5/0784;C12N5/10;C12N15/117 |
主分类号 |
C12N15/63 |
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