AUTOMATED PAP SCREENING USING A PLURALITY OF BIOMARKERS AND MULTI-SPECTRAL IMAGING

摘要

An automated screening method for detecting abnormalities in a sample. The method includes steps of staining a sample with a histologic or cytologic stain for transmission light microscopy to provide a stained sample; exposing the stained sample to a plurality of differentially-labeled biomarkers, wherein each of the biomarkers is labeled with a distinct transmission stain or fluorescence probe; at a first location, generating at least one multi-spectral image of the stained sample using signals obtained from the plurality of differentially-labeled biomarkers and the histologic or cytologic stain; and automatically determining whether the first location requires further pathologist review or interpretation.