| 摘要 |
A method of culturing human embryonic stem cells (hESC) whilst maintaining Stemness is claimed, the method comprising seeding hESC onto an uncoated fibre scaffold and incubating the hESC loaded fibre scaffold in a gas atmosphere in which the percentage of oxygen is less than the percentage of oxygen in air. The fibres may be electrospun fibres and the percentage oxygen may be in the range 0.25% to 20 %, preferably 0.5 % to 10 %. Cells obtained by such methods, implants containing such cells and medical methods using such cells are also disclosed. |
