发明名称 |
Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof |
摘要 |
The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal. |
申请公布号 |
US8653249(B2) |
申请公布日期 |
2014.02.18 |
申请号 |
US20090918776 |
申请日期 |
2009.02.27 |
申请人 |
SUZUKI YASUHIKO;YAMAMOTO KEIICHI;TAHARA HIROSHI;SUZUKI YUSUKE;FUSO PHARMACEUTICAL INDUSTRIES, LTD.;NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY |
发明人 |
SUZUKI YASUHIKO;YAMAMOTO KEIICHI;TAHARA HIROSHI;SUZUKI YUSUKE |
分类号 |
C07H21/04;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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