摘要 |
A single stranded oligonucleotide having substantially no secondary structure and formed of nucleotide residues wherein two or more internal nucleotide residues are labelled with a fluorophore without an associated quencher. Typically at least two of the nucleotide residues labelled with a fluorophore are separated by at least two unlabelled nucleotide residues. The oligonucleotides are useful in the investigation of target polynucleotide sequences. |