| 摘要 |
PURPOSE: An extraction method of collagen from chicken by-products is provided to be able to produce useful collagen by using chicken skin, and to be able to provide a functional material capable of generating an added-value utilizing waste resources and producing high quality food. CONSTITUTION: An extraction method of collagen from chicken by-products comprises (1) a step of washing and slicing the chicken skin; (2) a step of swelling the sliced chicken skin in step (1) by dipping a dipping solution of 10 times (w/v) the weight of the chicken skin and pH 1-13 at a room temperature; (3) a step of neutralizing the swollen chicken skin in step (2) to pH 5-7 by washing in the flowing water; (4) a step of extracting with hot-water by immersing the neutralized chicken skin in step (3) in a thermo-bath preset to 75-100deg.C; (5) a step of collecting gelatin sol by filtering the extracted effluent in step (4); (6) a step of gelating the collected gelatin sol in step (5) by keeping in a refrigerator at 3-5deg.C for 4-8 hours; and (7) a step of freezing and drying the gelatin gel from step (6) at -60 to -80deg.C. [Reference numerals] (AA) Trimmed chicken skin (size of 3x3 cm after removing fats and connective tissues); (BB) Swelling after soaking (pH 1-13, room temperature, 24 hours); (CC) Neutralization by using running water (pH 3-7, 48 hours); (DD) Discharge hot water after packing (75-100°C, 1 hour); (EE) Filtration (Nylon filter); (FF) Gelation (4±1°C, 6h); (GG) Powderization after freeze-drying |
