METHOD FOR IDENTIFYING A SPECIFIC BINDING INTERACTION BETWEEN TWO OR MORE POLYPEPTIDES

摘要

The present invention provides a method for identifying a specific binding interaction between a first polypeptide and a second polypeptide which comprises the following steps: (i) combining a) a first polysome which comprises the first polypeptide and a first tag with b) a second polysome which comprises the second polypeptide and a second tag; and (ii) investigating whether a complex forms between the first and second polysomes which comprises both the first tag and the second tag wherein the formation of a complex which comprises both the first and second tags indicates that the first polypeptide and second polypeptide specifically interact.