发明名称 |
Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate |
摘要 |
Provided is a ultra-high-sensitivity assay in which the assay can be made on a commonly used assay apparatus such as an absorptiometer and a plate reader or with naked eyes. The high-sensitivity assay in which the assay can be made on a commonly used assay apparatus or with naked eyes can be provided by combining an enzyme cycling method using thio-NAD(P) as a coenzyme, a labeling enzyme and a substrate for the labeling enzyme optimally, and by amplifying thio-NAD(P)H, which is a signaling substance, exponentially and then quantifying the thio-NAD(P)H colorimetrically. |
申请公布号 |
EP2695948(A1) |
申请公布日期 |
2014.02.12 |
申请号 |
EP20120759947 |
申请日期 |
2012.03.23 |
申请人 |
ITO, ETSURO;WATABE, SATOSHI;NAKAISHI, KAZUNARI |
发明人 |
ITO, ETSURO |
分类号 |
C12Q1/00;C12Q1/42;C12Q1/68;G01N21/25;G01N21/78 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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